yabD [2019-04-04 13:25:08]

putative metal-dependent hydrolase

locus: BSU00390

pI: 4.81

mw: 29.08 kDa

protein length: 255 aa

gene length: 768 bp

function: unknown

product: putative metal-dependent hydrolase

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 47,706 48,473

The protein

Protein family

• [SW|Metallo-dependent hydrolases superfamily] (according to UniProt)

Structure

• [PDB|2GZX] (from Staphylococcus aureus, 64% identity)

Expression and Regulation

Operons

• genes: [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS]-[gene|00798BB5A769C464CC5FDDF7D9E41D30B473B9A8|yabD]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

  additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of [gene|DCBDD135F295B04FE7C7EB92E94638701CB320DD|metS] [Pubmed|27120414]

Biological materials

Mutant

• MGNA-B904 (yabD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1903 NBRP B. subtilis, Japan]
• BKE00390 ([gene|00798BB5A769C464CC5FDDF7D9E41D30B473B9A8|yabD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTGAAAAACTCCTTT, downstream forward: _UP4_TGACAAAAAACGCTAGCGGG
• BKK00390 ([gene|00798BB5A769C464CC5FDDF7D9E41D30B473B9A8|yabD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGTGAAAAACTCCTTT, downstream forward: _UP4_TGACAAAAAACGCTAGCGGG

References

• 10747959